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Jawaharlal Nehru Tropical Botanic Garden and Research Institute
Palode, Thiruvananthapuram, Kerala, India - 695562
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Dr. P.N. Krishnan
    Scientist - F & Head
Biotechnology and Bioinformatics Division
Phone:(O)0472-2869226
(R)0471-2374155
Fax: 91(0) 472 - 2869646
E-mail: peringattulli@yahoo.co.uk
 
 
 
 
Dr. P.N. Krishnan
     
Area of Interest : Plant Biotechnology, Seed Bank, Biodiversity Informatics & Plant Physiology
 
Honors and Awards Received
1
Technical expert and Mentor for the KSIDC proposed Biotechnology Incubation Centre at Life Science Park, Thiruvanathapuram. The project was formerly approved by BIRAP –DBT, Govt of India for funding at the tune of Rs. 18.00 cores.
2
Dr.P.N.Krishnan was nominated as External Member of the Screening Committee for Scientists selection and Internal Screening Committee for Assessment of Scientists at KFRI , Peechi, Trissur.
3
Dr.P.N.Krishnan was a Panel Expert on the concluding session of the International Workshop on Digital documentation of Biodiversity organized by WII, ZSI and BSI on 15-17 June 2011
4
Dr.P.N.Krishnan was invited as an expert by the Career Guidance and Placement Cell(CGPC) of Mohandas College of Engineering Technology for conducting a model interview for the final year students of Biotechnology and Biochemical Engineering on 4th June 2011
5
Dr.P.N.Krishnan was a member of the Evaluating Panel for Paper presentation competttion on 03-01-2012 Colloquim 2012, at Mohandas College of Engineering Technology in Biotechnology and Biochemical Engineering
6
Dr.P.N.Krishnan was nominated as External Member of the Screening Committee for Scientists selection at CESS, Thiruvananthapuram
7
Dr.P.N.Krishnan was nominated as Chairman of the Internal Screening Committee for Scientists Promotion of JNTBGRI
8
Dr.P.N.Krishnan was nominated as the Member of the Management Committee of JNTBGRI
9
Dr.P.N.Krishnan is appointed as Member, Editorial Board of “Biotechnology Research” from 25th August 2011
10
FIRST PRIZE for the oral presentation of paper “ Plant- info – Software for the Management of Biodiversity Information” in the technical session Bioinformatics and biosafty issues of transgenic crops in the ICAR National Symposium on Biotechnological Interventions for Improvement of Horticultural Crops- issues and Strategies at College of Horticulture, Kerala Agricultural University, Thrissur, on 10th –12th January 2005
11
One of the authors of the " HARI OHM ASHRAM PREIT BHAIKAKA INTER UNIVERSITY SAMARAK TRUST" award winning paper in Botany entitled "Distribution of glycosidases and acid invertase activities in relation to internode elongation growth in Pearl Millet " appeared in JOURNAL OF EXPERIMENTAL BOTANY VOL.37: 1406-1415 (1986).
12
UGC & CSIR Fellow during 1978-83
13
Third Rank in M.Sc. Examinations conducted by Saurashtra University, Rajkot during 1978.
14
First Class First in M.Sc. Experimental Biology of 1978.
15
Merit Scholarship 1976 and 1977 during M.Sc. course.
Professional affiliations
1
Life member – Indian Society for Plant Physiology
2
Life member – Indian Society for Conservation Biology
3
Life member – Indian society for Biological Chemists (India)
4
Life member – Indian Society for Biotechnology (India)
5
Member – International Society for Plant Morphologists
6
Examiner for Ph.D., M.Phil. and M.Sc. in Plant Sciences, Calicut University.
7
Member, Board of Studies in Bioinformatics, Calicut University 23-9-05 - continuing
8
Chairman, Board of Studies in Bioinformatics, Calicut University, from 5-2-07-to 29-3-2010.
9
Member, Faculty of Sciences, Calicut University, from 25-4-07 – to 29-3-2010.
10
Member, Technical Committee and Honorary Advisor, Gujarat Biotechnology Mission, Gandhinagar.
Following are the major thrust areas of the Plant Biotechnology and Bioinformatics division in which R&D activities are coordinated by Dr P N Krishnan, as Head of the Division
1
Standardizing propagation protocols for rare, endangered and economically important medicinal plants, orchid through tissue culture and reintroducing to the native habitat.
2
Commercial micropropagation and extension activities with elite orchids and anthuriums.
3
Agro-forestry related in vitro mass multiplication of Bamboo species, Calamus species and rare and endemic medicinal tree species.
4
In vitro and cryobank repository of medicinal and aromatic plants.
5
Bioprospecting of selected medicinal plants using genetic variability analysis, RAPD, ISSR and DNA sequencing.
6
Production of high value, low volume compounds in selected medicinal plants through hairy root cultures.
7
Micropropagation protocol development for endangered orchids and development of cryobank repository of pollens.
8
Collection of traditional varieties of ‘Musa’, propagation through tissue culture and evaluation of yield and disease resistance.
9
Development of Biochemical criteria for detecting seed viability and desiccation through antioxidant enzymes.
10
Developing physiological and biochemical criteria for identifying better biomass accumulators at seedling stages in tree species.
11
Bioinformatics Centre a net work programme sponsored by Department of Biotechnology developing softwares and biodiversity database organization on the line of the mandate of the Institute and extending training on bioinformatics to scientists and researchers.
12
Extending training in biotechnology to college students, research scholars and tissue culture techniques in Orchids and Anthuriums to unemployed youth for income generation through small-scale propagation units.
R& D Programmes handled by Dr P N Krishnan
1
1. In Ph.D. programme, "Physiology of Dwarfism in plants” the role of auxin oxidising and auxin protecting systems were analyzed in different pearl millet cultivars exhibiting wide range of plant height at maturity to understand the basic distribution pattern in different cultivars vis-vis physiology of dwarfism.
2
2. After joining as Scientist in TBGRI on 1983, the first assignment to Dr Krishnan was Scientist-in-Charge of the new garden site at Palode. During this period piloted initial developmental activities of the garden site as coordinating the developmental activities.
3
3. In 1984 Dr Krishnan was transferred to the newly constituted Plant Biotechnology Division of TBGRI. As second in line actively took part in establishment of Plant Biotechnology division and also associated with R&D and training programmes of the division.
4
4. Biochemical changes related to carbohydrate availability and utilization during protocorm formation in the leaf base segments of an orchid, Cymbidium hybrid was worked out.
5
5. In an another aspect, changes in the level of total lipids, soluble sugars, starch and proteins were evaluated during different growth stages of protocorm formation of in vitro grown embryos of the terrestrial orchid species Spathoglottis plicata Blume. In this during initial stages of protocorm formation, the stored lipids and protiens were used and at later stages accumulated starch during the second period of culture was used for organogenesis.
6
6. In vitro culture of anthers and production of haploids in Cassava: Cassava (Manihot esculenta Crantz.) is a perennial shrubs and an important tuber crop grown in Kerala. The main objective of the work was to develop a suitable anther/ pollen culture technique for haploid production. Specifically, the project intended to step down the chromosome number from 36-18 and produce haploid which in evolutionary terms may be a ‘ diploid’ as the Cassava has a basic chromosome number n = 9. Mixoploid calli were induced from anthers and pollen of Cassava (M4 variety) from flower buds of 1.0-3.0 mm size collected in the morning and cold- pretreated for 2-3 days in MB media containing 3% of sucrose, 10-20% CW, 2.0 mg/1 BA, 1.0mg/1 NAA and caesin hydrolysate (200mg/l).
7
7. Tissue culture propagation of medicinal plants: Micropropagation through apical and axillary meristems was standardized for conservation and large-scale propagation for commercial exploitation and utilization of Woodfordia fruticosa (L.) Kurtz., Trichopus zeylanicus Gaertn., Holostemma annulare (Roxb.) K. Schum., Janakia arayalpathra Joseph & Chandrasekharan., and Rouwolfia micrantha Hook.f.
8
In Rouwolfia micrantha Hook.f perennial woody laticiferous shrub, hairy root cultures were raised from the roots of in vitro grown plants for root biomass production and subsequent secondary metabolite production of which ajmalicine percentage was higher and can be used for upgradation using fermentor system.
9
Plant regeneration via somatic embryogenesis was achieved from in vitro-derived leaf base segments of K. galanga. Responses of leaf segments cultured in MS medium supplemented with Dicamba, 2,4,5-T, NAA and IAA confirmed the efficiency of synthetic auxins viz. Dicamba and 2,4,5-T to induce embryogenic callus cultures. Callus subcultured on MS medium supplemented with 0.54 or 1.07 µM NAA along with 2.22 - 6.66 µM BA produced embryoids of different stages of development on the entire surface of the callus, which on transfer to hormone-free medium developed into plantlets in 4-6 wk.
10
In addition, cryopreservation of shoot tips and somatic embryos of Kaempferia galanga Linn using encapsulation dehydration and vitrification method was developed. Protocol established holds promise for long term conservation of this endangered economically important plant.
11
Effect of selected heavy metals (Al, Cu, Cd &Fe) on seed germination, seedling growth, changes in primary metabolites, and enzymes related to reserve mobilization in laboratory conditions and growth and biomass production in field conditions were analyzed in fast growing tree species like Adenanthera pavonina, Cassia fistula, Pongamia pinnata and Luceanea leucocephala
12
Study on the structure, function and dynamics of the sacred grooves of Kerala and their conservation dealt with analysis on conservation status, floristic structure, vegetation structure, dry matter dynamism, biomass accumulation and productivity of ground vegetation, edaphology etc. Also the ecological and environmental significance of these protected forest patches and their conservation is highlighted on sustainable utilisation of biodiversity.
13
Survey and evaluation of promising tree species and identification of plus trees for high and efficient biomass production, photosynthesis and nitrogen utilization were identified form different populations of six tree species from Southern Western Ghats of Kerala. The selected plus trees can be used for further upgradation and sustainable utilization on the line of conservation.
14
National Gene Bank Project on Medicinal and Aromatic Plants was extended for the 10th plan period (2002-2007) by Dept. of Biotechnology, Govt. of India based on the objectives on Exploration and Germplasm collection, development of field gene bank, seed bank, meristem bank, cryobank and morphological, cytological, chemical and molecular characterization of selected target species of peninsular India. As Principal co investigator of the project coordinated and supervised the activities at Biotechnology Division. During the period, 56 accessions belonging to 36 species were maintained through fresh culture initiation or subculture. The cultures were usually maintained in the rooting media and documented. In vitro multiplication was tested in 37 species using available protocol or using a few appropriations in media combination. A total of 35 accessions belonging to 22 species are maintained in the cryobank in the form of seeds, shoot tips or zygotic embryos. Genetic diversity analysis of Costus speciosus, Decalepis arayalpathra and Trichopus zeylanicus were done as part of the National Gene Bank for Medicinal and Aromatic Plants. The work is continued with the support of the institute funding.
15
Camptothecin(CPT) was isolated and quantified in four species of Ophiorrhiza,viz. Ophiorrhiza mungo (0.04%), O. eriantha (0.02%), O. nairii (0.01%) and O. prostrate (0.0065%) collected from different localities of Western Ghats. Tissue culture protocol was developed for Ophiorrhiza prostrata and O. eriantha in appropriate medium. Multiplication of shoots (12.5±2.33) was achieved through subculture of shoots in medium containing 0.5 mgl-1 BAP in 3 week. The shoots so produced were used for further multiplication, field establishment and isolation of the compound (CPT).
16
Molecular characterization and variability analysis of two selected plant species under the DBT project on species recovery focuses on delisting of endemic RET species of the Western Ghats using biotechnological intervention which also encompasses the study of indices of population biology and distribution including intraspecfic variability at genetic levels. In Poeciloneuron pauciflorum18 samples were collected from the Western Ghats. Fifteen random primers were used for the estimation of intraspecific variation in P. pauciflorum. The similarity matrix developed using the WINDIST software showed that similarity index ranges from 0.63 to 0.92 with mean value of 0.78 thereby suggesting high levels of genetic variability in the species. Further analysis using more number of primers is in progress
17
The Genetic Similarity matrix (GS) in Coscinium developed using WinDist programme measures value ranging from 0.50 to 0.90 with overall genetic similarity shared by all the 31 individuals of as 0.77 indicating wide variability within the species.
18
Development of sex specific markers in Coscinium fenestratum Pooled male and female DNA samples have been amplified with set of RAPD markers which resulted in the identification of unique bands specific to male and female plants. The bands were eluted out using the gel extraction kit from M/s Sigma USA, following the standard procedure. Sequence characterization of these amplicons is in progress
19
Nine samples of Trichopus zeylanicus including the seven that were morphologically characterized were subjected to genetic variability analysis using ISSR. The mean GS was found to be 0.74.
20
Genetic diversity analysis of Costus speciosus(24 accessions), Decalepis arayalpathra (21 accessions) and Trichopus zeylanicus (6 Accessions) were done as part of the National Gene Bank for Medicinal and Aromatic Plants using RAPD and ISSR.
21
DNA barcoding of Piper (KSCSTE project) 13 accessions comprising 6 species of Piper viz P. nigrum, P. hymenophyllum, P. galeatum, P. barberi, P. trichostachyon and P. wightii were selected for the present investigation. Six primer pairs complementary to three target sites (rpob, rpoc and matk) were analyzed in all the thirteen accessions. Amplicons ranging from 400 to 800 bp obtained were sequence characterized. It was found that percentage nucleotide diversity with in species was less (ranging from 0 to 0.49%) while between species it has gone up to 2.72%. However, in certain cases intraspecific nucleotide diversity was even more than interspecific diversity. Analysis of more number of samples seems to be needed to understand the SNP distribution and subsequent nucleotide polymorphism in this species.
22
Dr P N Krishnan took charge of the Coordinatorship of the Bioinformatics Centre of TBGRI sanctioned by DBT on 2001. The Centre functions on the mandate to organize a centralized digital data base including all information related to biodiversity of India and sharing these informations for augmenting R&D leading to conservation and sustainable utilization of the plant genetic wealth and economic growth of the nation. Infrastructure for the centre was developed on the line of communication facilities, computers, servers and leased line. Centre has developed the website of TBGRI, bioinformatics centre and managing the same.
23
Fungal database - Meliolales – a database offers information on 400 species of Indian Meliolaceous fungi (Black mildews). CD and Online version is available (www.tbgri.in).
24
Wild ornamentals of Western Ghats - a database offers information on 150 wild plants of ornamental value.
25
Database on Endemic plants of India, which comprises information about 1310 plant species made available on the web.
26
Plant info: Enrich the database by adding data of 250 plants. www.tbgri.in/plantinfo
27
Garden info TBGRI: database offers the primary data of 495 plants that are conserved in the Arboretum of TBGRI. www.tbgri.in/gardeninfo
28
Seed Pack TBGRI: database comprises primary data of 367 accessions. www.tbgri.in/seedpack
29
Sacred groves of Kerala version I: database offers information regarding 140 sacred groves of Trissur, Kottayam and Thiruvanthapuram districts of Kerala www.tbgri.in/sacredgroveonline
30
Litfriend -software for the management of literature.
31
Fungal data tool - an interface tool for the management of data of Black Mildews. www.tbgri.in/fungi
32
BTISNet Publication and Library – tool developed for the Bioinformatics Apex body, Department of Biotechnology, Govt. of India for the network of publication and library data of the 64 BTIS centers distributed all over India (www.tbgri.in/bioinfopub/).
33
Developed software application package for digitizing herbarium specimens and data, Infrastructure facilities like server, scanner and network has been established for the documentation of herbarium data.
34
On the line of validation of the efficacy of drug activity of plant derived biomolecules used in Indian traditional medicine through in silico methods screening of snake anti-venom compounds in Indian medicinal plants were initiated
35
Dr Krishnan is holding the charge of the seed bank of TBGRI 2001-2006 and coordinated all activities of the seed bank including R&D related to the storage. During the period initiated and streamlined the seed biology studies starting from flowering, fruiting, seeding, seed maturation, seed germination, viability, dormancy and storage aspects of rare, endemic and endangered species on the line of conservation linked sustainable utilization.
36
Apart from regular local collections from the garden flora, as a part of the National Gene Bank project on the Medicinal and Aromatic Plants, seed accessions from all of peninsular India were also collected for representing genetic variability at species level. A total of 113 accessions belonging to 38 species were deposited in the seed bank as part of National Gene Bank facility for Medicinal and Aromatic Plants. Detailed studies on seed character, seed germination, storage, viability and dormancy breaking if any were carried out in 14 selected species.
37
Ministry of Environment and Forests, Govt. of India funded project on seed bank, namely “Collection of seeds of listed endemic, endangered plants, seed bank and conservation in the field”, and also could get fund for the infrastructure development for the cold room and storage facility.
38
Updated the seed bank INDEX SEMINUM on 2003 published and circulated.
Detailed studies on desiccation and its effects on germination, viability and changes in content of free radical scavenging systems like peroxidase, polyphenol oxidise, SOD, together leachate conductivity with macromolecular changes were worked out on seeds of the endemic trees, Hopea parviflora and Vateria indica. On the basis of results it is inferred that decreasing concentration of oligosaccharides (raffinose, sucrose and stachyose) during desiccation affect the membrane stability of the seeds resulting the loss of vetrification that cause desiccation sensitivity in Hopea parviflora and Vateria indica. The increased level of lipid peroxidation and inverse relationship with germination, increased phenolic content during desiccation results to an enhanced membrane permeability there by leakage of solutes result the loss of viability in both the species. The study further shown that metabolism of sugars, proteins and lipids increased together with lipid peroxidation, activities of free radical scavenching enzymes like PO, PPO, CAT, electrolyte leakage, phenol accumulation etc. during desiccation of seeds may be considered as indicators of detecting seed viability.
39
The effect of Cadmium on seed germination, dry weight change, seedling vigor and bioaccumulation was studied in Vigna mungo under laboratory conditions. Histochemical studies were carried out to localize Cd, loss of membrane integrity, callose production and lipid peroxidation. Changes in primary metabolites , L-proline, hydrogen peroxide, ascorbate, non protein thiols and reduced glutathione(RP-HPLC) were analysed. Variation in activities of amylase and protease enzymes, root antioxidant/defense enzymes PER, CAT, SOD,PAL and PPO were also analysed.
40
Since April 2004 Institute has given an additional charge to Dr Krishnan to develop the Saraswathi Thangavelu Extension Centre of TBGRI at Puthenthoppu, Kaniyapuram.
41
The centre has established cultivation demonstration plots of orchid. Currently about 10,000 orchids cuttings were planted in the demonstration plots. In addition, different varieties of anthurims and other ornamentals are maintained. Following plants were introduced during the period.
42
Developed Web-site of the centre and hoisted.
43
Plants found in the centre were identified with the help of taxonomists and incorporated in the web site.
44
Compost making using the organic waste has been successfully practiced at Puthenthope campus.
45
Infrastructure like sprinklers and other requirements for the land was installed
46
The boundary wall construction in three sides main centre and two sides of the 2 acre plot has been completed.
47
An additional building was constructed behind the main building to accommodate the store room for garden implements, gardeners and kitchen facility.
48
Renovated the shade net house to accommodate anthuriums and shade loving plants. Renovated the main building and street lights were installed up to the gate.
49
Routine agricultural practice for the coconut trees and other horticultural plants of the main centre was initiated.
50
Standardization of Agrotechnological Package of Practices and Establishment of Demonstration Plots for Commercial Cultivation of Plectranthus vettiveroides”. – NMPB sponsored project: This externally funded field oriented programe is implemented at Puthenthope campus. The germplasm collected from 5 localities are maintained in the centre. Constructed a nursery shed at two acre plot sub centre. Identified the pathogen causing disease and standardized the remedial measures and field management of the crop. Standardized soil nutrient requirements both organic and chemical, best season for optimal growth and higher biomass production. Identified the weeds and its eradication. As the root is the raw material, effective harvesting technology is standardized to get maximum root biomass without loss.
Seminar organized
1
The Bioinformatics Centre jointly with C-DIT, Thiruvananthapuram organized a National workshop on “Bioinformatics Tools, Techniques and Algorithms” from 10th to 12th July 2002 at Thiruvananthapuram.
2
Convened one day National Seminar on “Recent Developments in Biotechnology” at TBGRI on 24th July 2002.
3
Organized a National Seminar on “Bioinformatics and Biodiversity Data Management“ from 15th to 17th May 2003 with financial assistance from Department of Biotechnology, Govt. of India, New Delhi.
4
Organized a National Seminar on “Sustainable utilization of Bioresources through Biotechnology and bioinformatics” on 18th and 19th December 2008 with financial assistance from DBT, Govt of India.
Workshop organized
1
Organized training for the scientific staff of TBGRI on “Computer as an aid for quality publications” from 18th to 20th October 2001.
2
Organized a National Workshop on "Biodiversity Informatics & Interlinking of Databases" from 24th to 28th October 2005 with financial assistance from Department of Biotechnology, Govt. of India, New Delhi.
3
3 Organized National workshop on "Biodiversity Database Organization and Its Management" from 16th to 20th July 2007 with financial assistance from Department of Biotechnology, Govt. of India, New Delhi.
4
4 Organized a National workshop on "Biodiversity Database Organization and Management" from 12th to 14th March, 2008. 15 participants including senior scientists, professors and college lectures participated in the workshop funded by Department of Biotechnology, Govt. of India, New Delhi.
5
5 Organised a 3 days National Workshop on 'Biodiversity Database Organization and Management', from 4th to 6th November, 2009, funded by Department of Biotechnology, Govt. of India, New Delhi.
6
Workshop on "Bioinformatics Tools: Sequence analysis, molecular visualization and docking", organized by Bioinformatics Centre at Puthenthope from 4th to 7th January, 2011 with financial support of DBT, Govt. of India.
7
Workshop on Statistics in research, intellectual property rights and patent law", organized by Bioinformatics Centre at Puthenthope from 18th to 20th July, 2011 with financial support of DBT, Govt. of India.
8
8. National seminar on Intellectual property rights & patent law and Dr. M. Thangavelu memorial lectures, organized by Bioinformatics Centre at Puthenthope on 31st October,, 2011 with financial support of KSCSTE, Govt. of Kerala.
Training attended
1
Dr. P. N. Krishnan attended Research Management training organized by Ministry of Environment and Forests, Govt. of India at Administrative Staff College of India, Hyderabad from 8th to 12th July 2002.
 
Research Projects undertaken
1
Assistant Investigator - In vitro culture of anthers and production of haploids in Cassava -ICAR, New Delhi, 1987-1990, Rs. 2.00 lakhs.
2
Principal Investigator - Effect of Heavy metals on growth and biomass production in some fast growing tree species from Kerala. - STED, Kerala State, 1994-1997, Rs. 0.75 lakhs.
3
Principal Investigator - Study on the structure, function and dynamics of the sacred groves of Kerala and their conservation.- Ministry of Environment and Forests, Govt. of India , New Delhi, 1995-1998,Rs. 6.85 lakhs.
4
Principal Investigator - Survey, evaluation and selection of promising fast growing native tree species of Western Ghats for efficient biomass production.- Man & Biosphere Program, Ministry of Environment and Forests, Govt. of India, 1997-2000, Rs. 12.50 lakhs.
5
Principal Investigator - Seed biology studies on selected medicinal plants of shola forests in Southern Western Ghats. - Kerala Forest Department, 1998- 2001, Rs. 4.52 lakhs.
6
Co-ordinator - Bioinformatics Centre, Net- Work Program of Dept. of Biotechnology, Govt. of India, 2001- 2017 continuing, Rs. 165.00 lakhs
7
Principal Investigator - Seed bank Project, Assistance to Botanic Gardens, Ministry of Environment and Forests, Govt. of India 2003- 2007, Rs 8.00 lakhs.
8
Co-Investigator - National Gene Bank for Medicinal and Aromatic plants, Dept. of Biotechnology, Govt. of India,.2002-2007, Rs. 95.00 lakhs
9
Principal Investigator: Standardization of agro-technological package of practices and establishment of demonstration plots for commercial cultivation of Plectranthus vettiveroids . National Medicinal Plant Board, Govt. of India 2007-2010, Rs. 23 lakhs.
10
Principal Investigator-Establishment of a centre for DNA barcoding of life forms for the identification and documentation of indigenous species of the Western Ghats in Kerala state. KSCSTE 2008-09, Rs 30.00 lakhs.
11
Co-Investigator-National programme on recovery of red-listed species (Coscinium fenestratum, Paphiopedilum druryi, Dialium travancoricum and Paeciloneuron pauciflorum) through application of biotechnological tools- DBT, Govt. of India.2007-2012 Rs 24.00 lakhs
12
Principal Investigator Tissue culture Multiplication of Screwpine. KSIDC, Govt. of Kerala. 2010-2013, Rs 17.085 lakhs
13
Principal Investigator: Integrated development of Peppara dam area through activities for plant resource enrichment, eco-restoration and tribal livelihood enhancement, SC&ST Department, Kerala State, 2009-2012, Rs 158.00 lakhs.
List of Publications
Books/Manuals authored/edited
1
1. Sreekumar, S, Biju, C.K. M. Raveendran and Krishnan, P. N. (2007). Training manual: Biological database organization in Microsoft Access, published by TBGRI (500 copies).
2
2. Sreekumar, S, Biju, C.K. and Krishnan, P.N. (2008). A booklet: DNA Barcoding (Malayalam) published by TBGRI (1000 copies).
3
3. A brochure highlighting Bioinformatics Centre’s activities (2008) (1000 copies)
4
4. Sreekumar S, Biju CK, William Decruze and P.N. Krishnan (eds.), Sustainable Utilisation of Bioresources through Biotechnology and Bioinformatics’ (2008) National Seminar Extended Abstracts, Published by TBGRI, Thiruvananthapuram. pp. 1 - 100.
Papers published
1
Rama Rao N, A R Jadeja, P N Krishnan, S C Naithani and Y D Singh (1981) : Interference of pottassium with cytokinin bioassay based on cucumber cotyledon expansion. Beitr. Biol. Pflanzen. 55: 189-194.
2
---Naithani S C, N Rama Rao, P N Krishnan and Y D Singh (1981) : Changes in o-diphenol oxidase activity during fibre development in cotton. Ann. Bot. 48 : 379-385..
3
Naithani S C , N Rama Rao, P N Krishnan and Y D Singh (1982) : Role of pottasium in kinentin and gibberellic acid induced peroxidase in isolated cucumber cotyledons. Beitr. Biol. Pflanzen. 57 : 221-228.
4
Chanda S V, A K Joshi, P N Krishnan, P P Vaishnav and Y D Singh (1986) : Distribution of Indole acetic acid oxidase, peroxidase and esterase activities in relation to elongation growth in pearl Millet internode. J. Plant Physiol. 122 : 373-383.
5
Joshi A K, S V Chanda, P N Krishnan, P P Vaishnav and Y D Singh (1986) : Chlorophyll content, Hill reaction and PEP case activity in relation to hybrid vigour in Pearl Millet during early seedling growth. J. Agro. Crop. Sci. 157 : 1-12.
6
Joshi A K, S V Chanda, P N Krishnan, P P Vaishnav and Y D Singh (1986) : Seedling Peroxidase and IAA oxidase activities in relation to hybrid vigour in pearl Millet. (Pennisetum americanum L., Leeke). J. Agro. Corp. Sci. 157 : 156-168.
7
Chanda S V, A K Joshi, P N Krishnan, and Y D Singh (1986) : Distribution of glycosidases and acid invertase activities in relation to elongation growth in Pearl Millet Internode J. Exp. Botany 37 : 1406-1415.
8
Chanda S V, A K Joshi, P N Krishnan, and Y D Singh (1987) : In vivo nitrate reductase activity in leaves of Pearl Millet, Pennisetum americanum (L) Leeke. Aust. J. Plant Physiol. 14 : 123-134.
9
Krishnan P N and Y D Singh (1988) : Endosperm amylase activity in Pearl Millet seedlings in relation to dwarfism. Current Sci. 57 : 1302-1304.
10
Krishnan P N (1990) : Nitrate uptake and nitrate reductase activity in relation to dwarfism in Pearl Millet. Proc. IInd Kerala Science Cong. (Ed. N. Balakrishnan Nair) pp. 69-71.
11
Gopalan G, P N Krishnan and S Seeni (1992) : Levels of starch and *-amylase during protocorn-like-body formation in foliar explants of a Cymbidium hybrid. Indi. J. Experi. Biol. 30 : 796-800.
12
Krishnan P N, PG Latha and S Seeni (1993) : Biochemical changes during protocorm formation from in vitro grown embryos of Spathoghottis plicata Blume. J. Orchi. Soc. India. 7 : 87-91.
13
Krishnan P N and P A Jose (1994) : Biomass cultivation – A promising source for the productivity in Kerala. Proc. 3rd Swadeshi Science Congress (Ed. K.S.M. Panikkar) Swadeshi Science Congress Movement, Kerala, pp 11-13.
14
Rajendra Prasad M, V Sarojini Menon, P N Krishnan and P Pushpangadan (1994) : Sacred Groves – A nutrient and water resource for Kerala. International Congress on Kerala Studies, Trivandrum pp 33-34.
15
Krishnan P N and M Rajendra Prasad (1994) : In vivo nitrate reductase activity in relation to leaf age and biomolecules in early seedlings of Adenanthera pavonina L. Beitr. Biol. Pflanzen 68 : 343-349.
16
Krishnan P N, S Seeni (1994) : Rapid micropropagation of Woodfordia fruticosa (L) Kurz. (Lythraceae) a rare medicinal plant. Plant Cell Reports 14 : 55-58.
17
Krishnan P N, C G Sudha, S Seeni (1995) : Rapid propagation through shoot tip culture of Trichopus zeylanicus Gaertn. a rare ethnomedicinal plant. Plant Cell Reports 14 : 708-711.
18
Abraham A, P N Krishnan and S Seeni (1995) : Induction of Androgenesis, callus formation and root differentiation in anther culture of cassava (Manihot esculenta Crantz.). Indi. J. Exp. Biol. 33 : 186-189.
19
Jose PA, Jacob Thomas and P N Krishnan (1995) : Vegetative propagation of Ochreinuaclea missionis (Wall ex G. Don) Ridsd. a rare, threatened tree species of Western Ghats. Indian Forester 121 : 1159-1164.
20
Krishnan P N (1995) : IAA Oxidising systems in relation to dwarfism in Pearl millet. Proc. IVth Swadeshi Science Congress, Ernakulam pp. 40-43.
21
Krishnan P N, S V Chanda and Y D Singh (1996) : Nitrate reductase activity in relation to dwarfism in Pearl millet. J. Sci. Agri. Research. 57 (203) :55-68.
22
Vijayakumar K and P N Krishnan (1996) : Effect of Aluminium on growth and primary metabolites in early seedlings of Adenanthera pavonina L. – A fast growing tree from Kerala. Proc. 8th Kerala Science Congress, Kochi, (ed. P.K. Iyengar). pp. 92-96.
23
Hosagoudar V B, T K Abraham, P N Krishnan and K. Vijayakumar (1997) : Biochemical changes in the leaves of Ebony tree affected with black mildew. Indian Phytopathol. 50 (3) : 438-440.
24
Vijayakumar K and P N Krishnan (1997) : Comparative study on the effect of Iron on early seedling growth in two native and one exotic fast growing tree species. Proc. 9th Kerala Science Congress, Thiruvananthapuram (ed. P K Iyengar) pp 90-93.
25
----- 1987. A new tar spot disease on Aporusa lindleyana (Wight) Baill. from Idukki, Kerala, India. Ibid. 11: 185-187.
26
Hosagoudar V B, P N Krishnan and T K Abraham (1997) : Biochemical changes in the sandal wood treee infected with Asterina conjesta Cook. New Botanist 24:27-32.
27
Rajendra Prasad M, P N Krishnan and P Pushpangadan (1997) : Role of sacred groves in conservation of biodiversity and protection of the environment. Kurukshetra XLVI (3): 19-22.
28
Sudha C G, P N Krishnan and P Pushpangadan (1998) : In vitro propagation of Holostemma annulare (Roxb.) K. Schum., a rare medicinal plant. In Vitro Cell & Dev. Biol. 34 (1) : 57-63.
29
Bagyanarayana, G., Subbalakshmi, G., Ramachar, P. and V.B. Hosagoudar 1987. Nyssopsora schefflerae sp. nov. from India. Curr. Sci. 56: 1022-1023.
30
Rajendra Prasad M, P N Krishnan and P Pushpangadan (1998) : The role of sacred groves of Kerala on the sustainable management of nutrient and hydrological cycle. Proc. 10th Kerala Science Congress, Kozhikkode (Ed. A D Damodaran). pp. 387-390.
31
Pushpangadan P, M Rajendraprasad and P N Krishnan (1998) : Sacred groves of Kerala – A synthesis on the state of-art-of knowledge. In Conserving the Sacred for Biodiversity Management (eds. Ramakrishnan P S, K G Sexena and U M Chandrashekara) Oxford & IBH Publishing Company, New Delhi, pp 193-210.
32
Rajendra Prasad M, P N Krishnan and P Pushpangadan (1998) : The life form spectrum of the sacred groves – A functional tool to analyse the vegetation. Tropical Ecology 39 (2): 211 – 217
33
Sudha C G, P N Krishnan, S Seeni and P.Pushpangadan (2000) : Regeneration of plants from in vitro root segments of Holostemma annulare (Roxb.) K.Schum., a rare medicinal plant. Current Science 78 (4): 503 – 506.
34
Rajendra Prasad M, P N Krishnan and P Pushpangadan (2000) : The vegetational characterisation and litter dynamism of the sacred groves of Kerala (South-West India) . J. Tropical Forest Science 12(2):320-335.
35
P N Krishnan and Rajendra Prasad M (2000):Changes in growth and physiological attributes in Adenanthera pavonina L. Saplings grown in normal sunlight and shade. Indian J. Plant Physiol. 5(1): 47-51.
36
Anil Kumar C, Krishnan P. N and Nabeesa Salim (2000): Seed viability of Syzygium aromaticum (L.) Merill & Perry during storage. Proc. Centennial Conference on Spices and Aromatic plants, 20-23 September 2000, Kozhikode, Indian Society for Spices pp. 55-59.
37
P N Krishnan and K Vijayakumar (2000) : Effect of Aluminium and copper on growth and biochemical changes in seedlings of Adenanthera pavonina L., a fast growing multipurpose tree species. Ecol. Env. & Cons. 6(4):429-434.
38
P N Krishnan, SV Chanda and Y D Singh (2000) :The role of auxin oxidising and protecting systems in the physiology of dwarfism of Pearl Millet. J.Sci Agric.Research 61. 214(4) :13-25.
39
Anilkumar C, K P Babu and P N Krishnan (2002) : Dormancy mechanism and effects of treatments on the germination of Garcina gummi-gutta (Clusiaceae) seeds. J. Tropical Forest Science 14(3):322-328.
40
Anilkumar C, K P Babu and P N Krishnan (2002) : Seed storage and viability of Myristica malabarica Lam. An endemic species of Southern western Ghats (India). Seed Sci. & Technol. 30:651-657.
41
Anilkumar C, M Kamarudeen and P N Krishnan (2003) : principles and practices in Seed bank – fruit harvesting, processing, seed collection, cleaning, drying and packing. In course material for the Training Programme on seed and nursery management, sponsored by FRLHT and Tamil Nadu Forest Department, pp 50-57.
42
Anilkumar C and P N Krishnan (2003) : management Practices in Seed Bank. In course material for the Training Programme on seed and nursery management, sponsored by FRLHT and Tamil Nadu Forest Department, pp 58-66.
43
Sudha C G, Krishnan P N, Pushpangadan P and S Seeni (2005) : In vitro propagation of Decalepis arayalpathra, a critically endangred ethnobotanical plant for ex-situ consevation – IN VITRO(Cellular and Developmental Biology-Plant) Accepted
44
Sreekumar, S. Safeer, PM, Biju, C.K., M. Raveendran and Krishnan, PN (2006): Workshop on biodiversity informatics and interlinking of databases. Current Science 90 (3): 280-281.
45
Preyesh Stephen, Sreekumar S, Biju C K, Krishnan P N (2006) MEDLIT: A Database Tool for the Management of Medicinal Plant Bibliographic Data. Indian Journal of Multidisciplinary Research. Vol. 2 (1): 81-86.
46
Chitra C R, S.Binu, C.Anilkumar and P.N.Krishnan (2006); Seed storage behaviour of Tinospora sinensis. Seed Research 34(2): 179-181
47
Sreekumar S, Biju C K , Krishnan P N (2007): LitFriend: A software package for personalized management of bibliographic information and its citation. Current Science 93 (4): 456-458
48
C A Kumar, C R Chitra, P N Krishnan, N Salim and G M Nair (2007): Effects of desiccation and temperature on the storage of Aegle marmelos seeds. Journal of Tropical Forest Science 19 (4); 236-239.
49
Krishnan, P.N., M. Raveendran, C.K. Biju and S. Sreekumar (2007) Kavu – Sacred landscapes of Kerala: Traditional Aspects of Biodiversity & Conservation Landscape No. 5(2): pp 27 -33.
50
Sabu T, Anilkumar C, Sham A, Nabeesa salim, Louis Jesudass, Mohanan N and P N Krishnan (2008): Seed maturity, germination and storage studies on an endemic tree- Humboltia decurrence Bedd.Ex Oliver. Indian Forester, 134(2): 203-211.
51
Sreedevi S, P N Krishnan and P Pushpangadan (2008) Cadmium induced oxidative damage and antioxidant responses in roots of Black gram [ Vigna mungo (L.) Hepper] Indian J Plant Physiology, !3(1):1-7.
52
Sreekumar S, Biju C K and P N Krishnan (2008): Biodiversity information resources available at Tropical Botanic Garden and Reseach Institute. Biobytes Vol. 3 :21-27
53
Preetha T S, Hemanthkumar A S, William Decruse S, Krishnan P N and S Seeni 2008. Effect of synthetic auxins on somatic embryogenesis from leaf-derived callus of Kaempferia galanga L. – an endangered medicinal plant. Phytomorphology 58(3&4) 117-124.
54
Preetha TS, Hemanthakumar AS and Krishnan PN (2009) Cryopreservation – An effective tool for the long-term conservation of Kaempferia galanga Linn., an endangered high value medicinal plant. Proccdings of the 21st Kerala Science Congress, 28-31 January 2009, Kollam, Organised by KSCSTE and TBGRI, pp. 602-604.
55
Sreekumar S, Biju CK and Krishnan PN (2008) Biodiversity Informatics: species identification and documentation. Proc. Nat. Sem. On Recent Trends in Biodiversity Conservation and Documentation, organised by M.G. Govt. Arts College, Mahe, 6 and 7 November, 2008. pp. 21-23.
56
Sreekumar S, Biju CK and Krishnan PN (2008) DNA barcoding of life forms: An emerging tool for rapid identification and documentation of species diversity. Training programme organized by CPCRI on ‘Principles and applications of Bioinformatics’. 3-7 November, 2008. pp. 41-47.
57
Preetha TS, AS Hemanthakumar, PN Krishnan and S. Seeni (2008) Long-term in vitro conservation of Kaempferia galanga L. through somatic embryo cryopreservation. National Seminar on Sustainable utilization of Bioresources, 18th and 19th December 2008, TBGRI, Thiruvananthapuram, Extended Abstract pp. 59-65. (Awarded second prize for best paper presentation).
58
Sam P Mathew , Radha RK , Krishnan PN and Seeni S(2010): Phytogeography of Andaman Nicobar Islands with special reference to extended distribution of Hornstedtia fenzlii (Kurz) K. Schum. Current Science, 98(7), 905-907
59
Radha R K, William Decruse S and Krishnan P N (2010): Cryopreservation of excised embryonic axes of Nothapodytes nimmoniana (Graham) Mebberly, a vulnerable tree species of the Western Ghats. Indian Journal of Plant Biotechnology, , 9: 435-437
60
Krishnan PN, Decruse S W and Radha R K (2011) Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology In Vitro Cellular & Developmental Biology - Plant 47 (1): 110-122 ( REVIEW ARTICLE)
61
Radha RK, Shereena SR, Divya K, Krishnan PN and Seeni S(2011) In vitro propagation of Rubia cordifolia, a medicinal plant. International Journal of Botany. 7(1) 90-96.
62
Padmesh P, Reji J V, Mukunthakumar S, Praveen G, Vineesh P S ,Reby S, Seeni S, Krishnan P N (2010) Analysis Of Genetic Diversity Among Selected Musa Cultivars Of Southern Western Ghats Through ISSR Markers. Res Bioscientia.(Accepted)
63
Mukunthakumar S, Praveen G, Vineesh P S, Reby S, Hari Kumar K, Seeni S, Krishnan PN (2010) Development of rapid in vitro propagation protocol for seedless diploid (AB) land race of Musa - ‘Ambalakadali’. International Journal of Academic Research 3: 1088-1095
64
Padmesh P, Sajan JS, Manikantan Menon K, Hemanthakumar AS, Pandurangan AG, Krishnan PN and Seeni S (2011) Analysis of Genetic Diversity in Poeciloneuron pauciflorum Bedd.?An Endemic Tree Species from the Western Ghats of India. American Journal of Plant Sciences, 2011, 2, 416-424
65
P. Padmesh, S. Mukunthakumar, P.S Vineesh, Reby Skaria, K. Harikumar and P.N. Krishnan (2012). Exploring wild genetic resources of Musa acuminata Colla distributed in the humid forests of southern Western Ghats of peninsular India using ISSR marker. Plant Cell Reports 31: 1591-1601DOI 10.1007/s00299-012-1273-5
66
Preetha TS,Hemanthkumar AS and P.N.Krishnan (2012) Effect of plant growth regulators on high frequency in vitro multiplication of a vulnerable woody medicinal climber Embelia ribes Burm. f.” Journal of Medicinal Plant Research Vol. 6(23), pp. 4011-4018, 21 June, 2012
67
A S Hemanthakumar, T S Preetha, P N Krishnan, S Seeni 2012. Utilization of zygotic embryos of an economic rattan palm Calamus thwaitesii Becc. (Arecaceae) for somaplant regeneration and cryobanking 3 Biotech DOI 10.1007/s13205-012-0083-3 August 2012
68
Anilkumar C, Chris Ipe, Bindu S Chtra C R, Mathew PJ and P N Krishnan 2012. Seed segregation of five South Indian Rauvolfia L. species by electrophoretic protein profiling. Current Science Vol.103(2): 138-140.
70
Krishnakumar G, Rameshkumar K B, Priya Srinivas, Satheeshkumar K and P N Krishnan 2012. Estimation of camptothecin and pharmacological evaluation of Ophiorrhiza prostrate D. Don and Ophiorrhiza mungos L. Asian pacific journal of Tropical Biomedicine S727-731.
71
P. Padmesh, J.S Sajan, K Manikantan, K.S Jayakumar and A.Subramoniam (2012). ISSR analysis reveals high intraspecific variation in Rauvolfia serpentina L.-A high value medicinal plant. Biochemical Systematics and Ecology 40: 192-197
Chapters in Book
1
Sreedevi S and Krishnan P N: detoxification of heavy metal induced oxidative stress in plants, enzymatic and non-enzymatic machanisms- a mini review In Oxidative Stress in Plants : causes, Cosequences and Tolerance Eds. Naser A Ajum, Shahid Umar and Altaf Ahemad, Chapter 7, pp 291-307 2012, IK International Publishing House, New Delhi.
 
  Reference:
   
  Prof. (Dr.) Kailash Paliwal
Director,
Indian Institute of Advanced Research
Koba Institutional Area
Gandhinagar 382007
Gujarat
   
  Dr T Madhan Mohan
Advisor
Department of Biotechnology
Ministry of Science and Technology
Block No 2, C G O Complex
Lodhi Road, New Delhi -110 003
     
   
Sunday, March 24, 2013
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