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Jawaharlal Nehru Tropical Botanic Garden and Research Institute
Palode, Thiruvananthapuram, Kerala, India - 695562
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Dr. S. William Decruse
    Scientist - C
Biotechnology and Bioinformatics Division
Phone: (O) 0472-2869226/626/622
Mob: 9497010229
E-mail: willdic@rediffmail.com
 
 
 
 
Dr. S. William Decruse
     
Area of Interest
:
Cryopreservation and micropropagation of plants
Research Highlights
 
  • Orchid micropropagation and reintroduction carried out in 5 species
     
  • Micropropagation of medicinal plant carried out in 7 species
     
  • In vitro conservation of medicinal plant carried out in 5 species
     
  • Established a cryopreservation laboratory
     
  • Cryopreservation protocol using shoot tips standardized in one species
     
  • Seed Cryopreservation of medicinal plants carried out in 16 species
     
  • Zygotic Embryo Cryopreservation of medicinal plants Standardized in 5 species
     
  • A cryobank of Western Ghats' orchids established for 18 species
     
  • Orchid seed cryopreservation succeeded in 20species
     
  • Orchid pollinia cryopreservation succeeded in 25 species
     
  • Studies of plant distribution modeling and restoration through micropropagation initiated in 3 endangered orchids (Eulophia cullenii, Vanda wightii, V. thwaitesii) and two rattans (Calamus shendurunii, C. wightii) of Western Ghats
    Projects Handled
    National Gene for Medicinal and Aromatic Plants funded by DBT, Govt. of India Co-PI 2002-2007
    Micropropagation and Cultivation of most sought after medicinal herbs used in Ayurveda and allied systems with people's participation. Project funded by DST, Govt. of India for three years.
    Principal Investigator
    2003-2007
    Establishment of a Seed and Pollen Cryobank for ex situ conservation and sustainable utilization of Orchids of Western Ghats. Project funded by DBT, Govt. of India for three years. Co-PI 2003-2006

    National programme on recovery of red-listed species (Coscinium fenestratum, Paphiopedilum druryi, Dialium travancoricum and Poeciloneuron pauciflorum) through application of biotechnology tools. Project funded by DBT, Govt. of India for five years. Co-PI 2006-2011

    Integrated Development of Peppara Dam Area. Project funded by STDD, Government of Kerala Co-PI 200309-2013

    Conservation of Vanda thwaitisii, V. wightii and Eulophia cullenii three endangaered orchids of Western Ghats through micropropagation and restoration with tribal participation. Project funded by DBT, Government of India for five years. Principal Investigator 2010-2015

    Conservation of Calamus shendurunii Anto, Renuka and Sreekumar and C. wightii Griff. Two endangered and endemic rattans of Western Ghats through micropropagation, reintroduction and cryobanking. Project supported by State Forest Department, Government of Kerala, 2012-16 (PI) Co-PI 2003-2006

    3 Establishment of a Seed and Pollen Cryobank for ex situ conservation and sustainable utilization of Orchids of Western Ghats. Project funded by DBT, Govt. of India for three years; 2003-2006, completed (Co-PI). Co-PI 2003-2006

    Publications
    Books
    :
    1
    Book Chapters
    :
    7
    Papers
    :
    18
    Gemeral articles
    :
    1
    Seminar Presentations
    :
    20
    M. Sc/B.Tech/BSc Projects Guided: 13
    1 Studies on in vitro multiplication, conservation and hairy root induction of three selected medicinal plants, viz; Rauvolfia beddomei, Holostemma annulare and Withania somnifera (MSc. Project by Sreejesh. P.S, School of Environmental Studies, Cochin University of Science and Technology)
    2 Seed Cryopreservation of Spathoglottis plicata Bl., a Terrestrial and Exquisite Orchid (MSc. Project by Ms. V.K. Gimsheena, Cochin University of Science and Technology).
    3 Pollen Cryopreservation of Spathoglottis plicata Bl., a terrestrial and Exquisite Orchid, (MSc. Project by Ms. Bindya Susan Mathew, Department of Biotechnology, PSG College of Arts and Science, Bharathiar University, Coimbatore)
    4 Influence of Plant Growth Regulators on Multiplication and Rooting from in vitro derived cultures of Rauvolfia serpentina Benth.ex kurz., an endangered medicinal plant (B.Tech Project by Miss C. Anusree, Department of Biotechnology and Biochemical Engineering, Mohandas College of Engineering and Technology, University of Kerala, Thiruvananthapuram).
    5 Effect of Paclobutrazol, a growth retardant on in vitro cultures of Kaempferia galanga L. and shoot tips subjected to cryopreservation (MSc. Project by Mr. Sreeraj .V, Department of Biotechnology, Nehru Arts and Science College, Bharathiar University, Coimbatore).
    6 Effect of Sodium Chloride induced stress on in vitro cultures of Kaempferia galanga L. and shoot tips subjected to cryopreservation (MSc. Project by Miss. Resmi AG, Department of Botany & Biotechnology, CMS College, Kottayam, Mahatma Gandhi University, Kottayam).
    7 Seed and Protocorm Cryopreservation of Vanda tessellata, an exquisite epiphytic orchid. Miss. Dhanya, Department of Botany & Biotechnology, CMS College, Kottayam, Mahatma Gandhi University, Kottayam (2008)
    8 In vitro multiplication studies on Rauvolfia beddomei Hook f. an endangered medicinal plant of Western Ghats”, (BSc Project by Miss. Arsha Philipose Babu, Mar Thoma College of Science and Technology, Chadayamangalam, University of Kerala)
    9 In vitro multiplication studies on Holostemma annulare (Roxb.) Schum, a threatened medicinal plant of Peninsular India (BSc Project by Miss. Reshma Raj, Mar Thoma College of Science and Technology, Chadayamangalam, University of Kerala)
    10 In vitro multiplication studies on Plumbago rosea L, a commercially important medicinal plant (BSc Project by Miss. Krishna S. Kumar, Mar Thoma College of Science and Technology, Chadayamangalam, University of Kerala)
    11 In vitro multiplication studies on Curcuma aromatica Salisb, an endangered medicinal plant (BSc Project by Miss. Pillai Preetha Premchandran, Mar Thoma College of Science and Technology, Chadayamangalam, University of Kerala)
    12 In vitro multiplication studies on Geophila reniformis D. Don, a threatened medicinal Technology, Chadayamangalam, University of Kerala)
    13 Micropropagation of Curcuma aromatica Salisb, an endangered medicinal plant (MSc project by Lakshmi R. Raj, Nehru Arts and Science College, Coimbatore affiliated to Bharathiar University )
    List of publications
      Research Papers  
    1 S. William Decruse and R. Kaleysa Raj (1992) Cytology and Sex determination of Setaria digitata (von Linstow 1906), bovine filarial parasite (Nematoda: Filarioidea). Cytologia, 57: 511-514
    2 S. William Decruse, S. Seeni and P. Pushpangadan, (1999) Effect of Cryopreservation on Seed germination of Selected rare medicinal plants of Peninsular India, Seed Science and Technology, 27:501-505).
    3 S. William Decruse, S. Seeni and P. Pushpangadan, (1999) Cryopreservation of alginate coated shoot tips of in vitro grown Holostemma annulare (Roxb.) K. Schum, an endangered medicinal plant: influence of preculture and DMSO treatment on survival and regeneration, Cryo-Letters, 20: 243-251
    4 A. Gangaprasad, S. William decruse, S. Seeni and V. Sarojini Menon (1999). Micropropagation and restoration of the endangered Indian daffodil orchid Ipsea malabarica (Reichb. f.) Hook. f., Lindleyana, 14: 38-46.
    5 S. William Decruse and S. Seeni. (2002) Ammonium nitrate in the culture medium influences regeneraton potential of cryopreserved shoot tips of Holostemma annulare, Cryoletters, 23: 55-50.
    6 S. William Decruse and S. Seeni. (2003) Seed Cryopreservation is a suitable storage procedure for a range of Piper species. Seed Science and Technology 33: 213-217
    S. William Decruse, A. Gangaprasad, S. Seeni and V. Sarojini Menon (2003), Micropropagation and ecorestoration of Vanda spathulata an exquisite orchid. Plant Cell Tissue and Organ Culture 72: 199-202.
    7 S. William Decruse, S. Seeni and G. M. Nair (2004) Preparative procedures and culture medium affect the success of cryostorage of Holostemma annulare shoot tips. Plant Cell Tissue and Org. Cult. 76: 179-182
    8 Krishnan PN, Decruse S W and Radha R K (2011) Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology In Vitro Cellular & Developmental Biology - Plant 47 (1): 110-122
    9 S. William Decruse and R. Kaleysa Raj.(1988) Excretory Secretory material from different sites of female reproductive tissue of filarial parasite Setaria digitata. Indian Journal of Experimental Biology, 26: 781-783
    10 S. William Decruse and R. Kaleysa Raj (1990). Histological studies on female Setaria digitata (von Linstow 1906), a filariae of bovine, Bos indicus. Proceedings of the Indian Academy of sciences (Anim.Sci.), 99: 103-112
    11 KP. Laladhas, S. William Decruse and R. Kaleysa Raj (1993) Synthesis and release of proteins homologous to excretory-secretory antigens during embryogenesis of Setaria digitata. Journal of Biosciences, 18: 311-318
    12 V. Sarojini Menon, S. Seeni, S. William Decruse and A. Gangaprasad. (1995) A preliminary study on the Habitat, Distribution and population density of Paphiopedilum druryi (Bedd.) Stein at Agasthyamalai in southern India. The Journal of the Orchid Society of India. 9: 55-67
    13 S. William Decruse, A. Gangaprasad, S. Seeni and V. Sarojini Menon (2003), A protocol for shoot multiplication from foliar meristem of Vanda spathulata (L.) Spreng. Indian J. Experimental Biology 41: 924-927
    14 A. Gangaprasad, S. William Decruse, S. Seeni and G.M. nair (2005). Micropropagation and ecorestoration of Decalepis arayalpathra (Joseph & Chandra.) Venter- an endemic and endangered ethnomedicinal plant of Western Ghats. Indian Journal of Biotechnology 4 265-270
    15 Preetha TS, Hemanthkumar AS, William Decruse S, Krishnan PN and Seeni S (2008) Effect of synthetic auxins on somatic embryogenesis from leaf-derived callus of Kaempferia galanga L. an endangered medicinal plant. Phytomorphology, 58: 117-124
    16 Radha R.K. S. William Decruse and P.N. Krishnan (2010) Cryopreservation of excised embryonic axes of Nothapodytes nimmoniana (Graham) Mebberly- A vulnerable medicinal tree species of the Western Ghats. Indian Journal of Biotechnology, 9: 435-437
    17 Joemon Jacob, C. R. Chitra, K. C. Kariyappa and S. William Decruse (2010) Fruit/seed morphology and germination characteristics in ten species of Calamus, J. Bamboo & Rattans, 9:73-78
      Booklet  
    1 S. William Decruse, Pathu oushadha sasyangalum avayude krishi reethiyum, published as a training manual as part of a DST sponsored project on medicinal plant cultivation, TBGRI, 2006
      Book Chapters  
    1 R. Kaleysa Raj, VS. Sugunan, S. William Decruse and J. John Bright (1991). Utility of Bovine filarial antigens in the Detection of Human filariasis. Procedings of the third Kerala science Congress pp. (STEC, Govt. Kerala) 222-223
    2 P. Pushpangadan, S. Seeni, Jacob Thomas, S. William Decruse, A. Mohandas, S.P. Mathew, A. Gangaprasad and R.K. Radha. (1999) Gene Bank for Medicinal and Aromatic Plants at Tropical Botanic Garden and Research Institute- A state of the Art Report. In: Medicinal and Aromatic Plants: Strategies and Technologies for Conservation. (Eds.) Salleh KM, Natesh S, Osman A and Kadir AA. (Forest Research Institute, Malaysia) pp. 63-71
    3 S. Seeni, A. Gangaprasad and S. William Decruse (2001). Species seedling flasks: An urgent need for ex situ conservation and sustainable economic utilization of the Western Ghats orchids. In: Orchids: Science and Commerce (eds) pPromila Pathak, RN. Sehgal, Navdeep shekhar, Madhu Sharma and Anil Sood. (Bishen singh Mahendra Pal Singh, Dehra Dun)
    4 A. Gangaprasad, S. William Decruse, and S. Seeni (2003) Orchids of Agasthyamala and their Conservation.In: Plant Diversity: Human Welfare and Conservation. (Eds.) M.K. Janarthanam and D. Narasimhan (Goa University)pp. 370-380
    5 S. William Decruse (2003). Cryopreservation with respect to seeds/Embryos. In: Perspectives of Seed and Nursery Management, Course material for the Training Programme on Seed and Nursery Management (Tropical Botanic Garden and Research Institute, Thiruvananthapuram) pp.67
    6 Sooriamuthu Seeni and S. William Decruse (2007), In vitro multiplication and restoration of selected RET plants of India, Proceedings of 3rd Global Botanic Garden Congress, 16-20 April 2007, China.
    7 S. Ganeshan, PE. Rajasekharan, S. Sasikumar and S. William Decruse (2008). Cryopreservation of Pollen In: Plant Cryopreservation: A Practical Guide (Ed) B.M. Reed (Springer) pp 443-464
    8 Sayujia S. Vargheese, S. William Decruse and P.N. Krishnan (2012) Pollinia Cryopreservation of Eulophia nuda Lindl. Procedings of the 24th Kerala Science Congress pp. (KSCSTE, Govt. Kerala) 230-232
      General Article  
    1 Ini Pachavellakkeniyum, Kerala Karshakan, FIB, Kerala, 2005
      Seminar Presentations  
    1 S. William Decruse and R. Kaleysa Raj (1988). Studies on secretory antigens associated with development of microfilariae in Setaria digitata Procedings of the first national seminar on frontiers in biology (Department of Biochemistry, University of Kerala) pp 101
    2 S. William Decruse and R. Kaleysa raj. (1989). Excretory-Secretory (ES) antigens related with embryogenesis in the bovine filarial parasite Setaria digitata. XVI annual conference and symposium on tropical immunology (Mahatma Gandhi Institute of Medical Science and technology, Sevagram)
    3 S. Seeni, PG. Latha, A. Gangaprasad, S. William Decruse and V. Sarojini Menon.(1991) National seminar on Biology, Improvement, Propagation and Commercialization of Indian Orchids (The Orchid society of India, IIHR, Bangalore)
    4 S. William Decruse, A. Gangaprasad, S. Seeni and V. Sarojini Menon. (1994) Nutritional requirements of selected epiphytic and terrestrial species seeds in vitro. National seminar on Impact of Plant Physiology on Indian Agriculture (Department of Plant Physiology, Agricultural College, Bapatla.)
    5 A. Gangaprasad, S. William Decruse, S. Seeni and V. Sarojini Menon. (1994) Sustainable Development and Economical Utilization of wild orchid resources of Kerala. International Congress on Kerala Studies (AKG Centre for Research and Studies.)
    6 A. Gangaprasad, S. William Decruse, S. Seeni and V. Sarojini Menon. (1995) Species Seedling and Mericlone Bottles for practical conservation and sustained economic utilization of the Western Ghats orchids. Seventh Kerala science Congress (STEC,,Kerala.)
    7 A.Gangaprasad, S.William Decruse, S. Seeni and V.SarojiniMenon. .(1996) Micropropagation and reintroduction of Ipsea malabarica and Vanda spathulata, two rare and exquisite orchids of Western Ghats. National Seminar on Conservation of endangered species and ecosystems. biotechnological and ecological approaches. (Banaras Hindu University, Varanasi)
    8 S. William Decruse, S. Seeni and P. Pushpangadan (1998) Slow growth in vitro conservation of selected medicinal plants from the Western Ghats, International Conference on Conservation of Tropical species, Communities and Ecosystems (TBGRI, Trivandrum, India).
    9 S. William Decruse, S. Seeni and P. Pushpangadan (1998) In vitro conservation of medicinal plants from the Western Ghats,. International conference on medicinal plants (FRLHT, Bangalore, India)
    10 S. William Decruse, A. Gangaprasad, S. Seeni and V. Sarojini Menon. (2000) Conservation through micropropagation of exquisite Vanda spathulata First meeting of the Indian Subcontinent Region Orchid Specialist Group (TBGRI, Palode, Thiruvananthapuram)
    11 Sarajith, S. Ajeeshkumar, S. William Decruse, A. Gangaprasad and G.M. Nair. Seed cryopreservation is effective for the long-term ex situ conservation of native orchids of Western Ghats. National Seminar on Biodiversity conservation, IAAT, December 2004, (TBGRI, Thiruvananthapuram)
    12 R.K. Radha, S. William Decruse, PN. Krishnan and G.M. Nair (2004) Zygotic embryo cryopreservation of Nothapodytes nimmoniana. National Seminar on Angiosperm Taxonamy and Annual conference of IAAT, December 2004, (TBGRI, Thiruvananthapuram)
    13 Radha R.K, William Decruse S, Krishnan PN, Seeni S, and Ganeshan S. (2006) Cryopreservation of embryonic axes of recalcitrant seed species Myristica malabarica Lam., a rare medicinal plant of the Southern Western Ghats. National Seminar on Plant Resources of Western Ghats, 7th and 8th December 2006, IISC, Bangalore.
    14 S. William Decruse and S. Seeni (2008) Micropropagation and restoration of Smithsonia maculata (Dalz.) Sald. National Seminar on Sustainable utilization of Bioresources, 18th and 19th December 2008, TBGRI, Thiruvananthapuram
    15 S. William Decruse, S. Shailajakumari and S. Seeni (2008) Micropropagation of Curcuma aromatica Salisb., an important medicinal plant used in Ayurveda
    National Seminar on Sustainable utilization of Bioresources, 18th and 19th December 2008, TBGRI, Thiruvananthapuram
    16 S. Ajeeshkumar and S. William Decruse (2008) Pollinia cryopreservation of Cymbidium bicolor and its utilization in breeding. National Seminar on Sustainable utilization of Bioresources, 18th and 19th December 2008, TBGRI, Thiruvananthapuram
    17 Reny N, Shailajakumari S, William Decruse S and Krishnan P.N.(2010) Micropropagation of Eulophia cullenii Wight (Blume), a critically endangered medicinal and exquisite orchid of Western Ghats for ecorestoration. Golden Jubilee National Symposium on Plant Diversity Utilization and Management, Department of Botany, University of Kerala, 27-29th May 2010
     
    Tuesday, March 26, 2013
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